| 质粒名称: | pTom20-mScarlet3-H (aka mYongHong) |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000760 |
| 产品用途: | Expresses Tom20-mScarlet3-H in mammalian cells |
| 存储实验室: | Zhifei Fu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pN1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 卡那霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | Tom20, mScarlet3-H |
| 物种来源: |
H.sapiens, Synthetic
|
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
A highly stable monomeric red fluorescent protein for advanced microscopy
Xiong H., Chang Q., Ding J., Wang S., Zhang W., Li Y., Wu Y., Lin P., Yang C., Liu M., Fang G., Yang Y., Xie J., Qi D., Jiang T., Fu W., Hu F., Chen Y., Yue R., Li Y., Cui Y., Li M., Fan S., Yang Y., Xu Y., Li D., Zhang F., Zhao H., Wu C., Zheng Q., Piatkevich KD., Fu Z.
Nat. Methods 2025 1–11 (2025)
|
pTom20-mScarlet3-H (aka mYongHong)
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pTom20-mScarlet3-H (aka mYongHong)载体质粒基本信息
pTom20-mScarlet3-H (aka mYongHong)质粒图谱载体图谱和pTom20-mScarlet3-H (aka mYongHong)载体序列质粒序列多克隆位点信息
载体图谱.png)
pTom20-mScarlet3-H (aka mYongHong)质粒载体简介
pTom20-mScarlet3-H (aka mYongHong)质粒序列载体序列
LOCUS pTom20-mScarlet3-H (aka mYongHong) 5095 bp DNA circular SYN 30-DEC-2024
DEFINITION TSN20241013-0571-00010_760_20241014-BAN4_C04_C04_i20.1.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5095)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 23, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5095
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 40..397
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 248..383
/label=SV40 ori
/note="SV40 origin of replication"
CDS 432..1226
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 1458..1505
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 1834..2422
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
enhancer 2597..2900
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 2901..3104
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
regulatory 3133..3142
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 3139..3573
/codon_start=1
/label=TOMM20
/translation="MVGRNSAIAAGVCGALFIGYCIYFDRKRRSDPNFKNRLRERRKKQ
KLAKERAGLSKLPDLKDAEAVQKFFLEEIQLGEELLAQGEYEKGVDHLTNAIAVCGQPQ
QLLQVLQQTLPPPVFQMLLTKLPTISQRIVSAQSLAEDDVE"
regulatory 3598..3607
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 3604..4293
/codon_start=1
/label=mScarlet3-H
/translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
LRVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYWKQSFPEGFKWERVMNFEDGGA
VSVAQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDVVLKGDIKH
ALRLKDGGRYLADFKTTYRAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHS
TGGSGGS"
polyA_signal 4419..4540
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(4547..5002)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter join(5029..5095,1..38)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 cctgataaat gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct
61 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
121 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
181 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
241 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
301 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
361 gtgaggaggc ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag
421 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
481 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
541 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
601 tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
661 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
721 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
781 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
841 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
901 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga
961 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
1021 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
1081 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
1141 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
1201 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc
1261 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg
1321 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
1381 ggagttcttc gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga
1441 aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt
1501 tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc
1561 ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg
1621 tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta
1681 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
1741 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
1801 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
1861 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
1921 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
1981 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
2041 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
2101 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
2161 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
2221 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
2281 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
2341 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
2401 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
2461 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
2521 tattaccgcc atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc
2581 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
2641 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
2701 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
2761 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
2821 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
2881 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
2941 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
3001 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
3061 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc
3121 agatccgcta gcgccaccat ggtgggtcgg aacagcgcca tcgccgccgg tgtatgcggg
3181 gcccttttca ttgggtactg catctacttc gaccgcaaaa gacgaagtga ccccaacttc
3241 aagaacaggc ttcgagaacg aagaaagaaa cagaagcttg ccaaggagag agctgggctt
3301 tccaagttac ctgaccttaa agatgctgaa gctgttcaga agttcttcct tgaagaaata
3361 cagcttggtg aagagttact agctcaaggt gaatatgaga agggcgtaga ccatctgaca
3421 aatgcaattg ctgtgtgtgg acagccacag cagttactgc aggtcttaca gcaaactctt
3481 ccaccaccag tgttccagat gcttctgact aagctcccaa caattagtca gagaattgta
3541 agtgctcaga gcttggctga agatgatgtg gaaggcggta gcggggatcc accggtcgcc
3601 accatggata gcaccgaggc agtgatcaag gagttcatgc ggttcaaggt gcacatggag
3661 ggctccatga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
3721 ggcacccaga ccgccaagct gagggtgacc aagggtggcc ccctgccctt ctcctgggac
3781 atcctgtccc ctcagttcat gtacggctcc agggccttca cgaagcaccc cgccgacatc
3841 cccgactact ggaagcagtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
3901 gaggacggcg gcgccgtgtc cgtggcccag gacacctccc tggaggacgg caccctgatc
3961 tacaaggtga agctccgcgg caccaacttc cctcctgacg gccccgtaat gcagaagaag
4021 acaatgggct gggaagcatc caccgagcgg ttgtaccccg aggacgtcgt gctgaagggc
4081 gacattaagc atgccctgcg cctgaaggac ggcggccggt acctggcgga cttcaagacc
4141 acctacaggg ccaagaagcc cgtgcagatg cccggcgcct tcaacatcga ccgcaagttg
4201 gacatcacat cccacaacga ggactacacc gtggtggaac agtacgaacg ctccgtggcc
4261 cgccactcca ccggcggctc cggtggctcc taataaagcg gccgcgactc tagatcataa
4321 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
4381 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
4441 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
4501 attctagttg tggtttgtcc aaactcatca atgtatctta aggcgtaaat tgtaagcgtt
4561 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
4621 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
4681 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
4741 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
4801 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
4861 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
4921 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
4981 aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
5041 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataac
//
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